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Rabbit Anti-GAP43 antibody (bs-0154R)
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產品編號 bs-0154R
英文名稱 GAP43
中文名稱 神經生長相關蛋白43抗體
別    名 Growth Associated Protein-43; Neuromodulin; Axonal membrane protein GAP 43; B-50; F1; GAP 43; Growth Associated Protein 43; Nerve Growth Related Peptide; Neural phosphoprotein B 50; Neuromodulin; GAP-43; pp46; NEUM_HUMAN; Protein F1; QtrA-11580; QtrA-13071.  
研究領域 腫瘤  免疫學  神經生物學  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human, Mouse, Rat, Chicken, Dog, 
產品應用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:200-800 (石蠟切片需做抗原修復)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 25/46kDa
細胞定位 細胞漿 細胞膜 細胞外基質 
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human GAP43:9-100/238 
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
產品介紹 The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Function:
This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction.

Subunit:
Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence.

Subcellular Location:
Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Cell projection, filopodium membrane; Peripheral membrane protein. Note=Cytoplasmic surface of growth cone and synaptic plasma membranes.

Post-translational modifications:
Phosphorylated at Ser-41 by PHK. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.
Palmitoylation by ARF6 is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2.

Similarity:
Belongs to the neuromodulin family.
Contains 1 IQ domain.

SWISS:
P06837

Gene ID:
2596

Database links:

Entrez Gene: 2596 Human

Entrez Gene: 14432 Mouse

Entrez Gene: 29423 Rat

GenBank: NP_002036 Human

Omim: 162060 Human

SwissProt: P17677 Human

SwissProt: P06837 Mouse

SwissProt: P07936 Rat

Unigene: 134974 Human

Unigene: 1222 Mouse

Unigene: 10928 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

神經生物學相關蛋白(Neurobiology);神經標志物
GAP43 (Growth associated protein-43)又稱作neuromodulin,是一個軸突膜蛋白,是一種神經特異性的蛋白質,參與神經細胞外生長及突觸發育形成和神經細胞再生。在神經元發育和再生過程中以高水平表達。能調解軸突延伸作用,改變細胞形態。作為細胞內信號,可大大增強與G蛋白偶聯的受體轉運作用。
神經生長相關蛋白-GAP-43和神經細胞黏附因子(neural cell adhesion molecule,NCAM)與突觸可塑性密切相關.GAP-43是一種神經細胞膜上的特異性磷蛋白,在神經發育和再生過程中呈現高表達,被作為突觸生長的標志物,有稱脊髓生長相關蛋白
GAP-43與CaM結合,參與G蛋白相互作用,神經遞質的釋放,作用于胞吞/胞吐過程,通過小囊溶合或誘導生長錐和突觸前末端的胞吞促進膜擴展,與海馬長時程增強密切相關.
神經細胞黏附因子是細胞表面糖蛋白大家族的成員之一,促進軸突生長,對長時記憶的保持有重要影響,同時,GAP-43對其具調節作用,廣泛而深入地研究GAP-43意義深遠。
產品圖片
Sample:
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-GAP43 (bs-0154R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25/46 kD
Observed band size: 22 kD
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-GAP43 (bs-0154R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25/46 kD
Observed band size: 22 kD
Sample:
Heart(Mouse) Lysate at 40 ug
Primary: Anti- GAP43(bs-0154R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25/46kDa kD
Observed band size: 25kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GAP-43 Polyclonal Antibody, Unconjugated(bs-0154R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAP43) Polyclonal Antibody, Unconjugated (bs-0154R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAP43) Polyclonal Antibody, Unconjugated (bs-0154R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Blank control (blue line): Hela cells (blue).
Primary Antibody (green line): Rabbit Anti-GAP43 antibody (bs-0154R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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